Thus, there should be an even difference between every concentration in serial dilution ELISA such as 1/3, 1/9, 1/27, and 1/81 reflecting a 3-fold difference between each dilution. But a dilution from 1/2 and 1/3 is only a 1.5-fold concentration difference, while 1/10 and 1/100 represent a 10-fold difference. For example, when examining an unknown polypeptide, dilutions of 1/2, 1/3,1/5, 1/10, and 1/100 may seem sound. For example, testing for SARS-CoV-2, the virus that causes COVID-19, is a major area where rapid testing is crucial.Serial dilution ELISA has to be calculated carefully, or very little useful information will be obtained. Serial dilution ELISA is an important part of testing whenever examining an unknown peptide (or protein molecule) or nucleic acid (or DNA/RNA molecule). With measurable reporter molecules attached to the antibodies, it is possible to measure the amount of protein or nucleic acid present. ELISA is based on the interaction of antibodies and antigens where the antibodies attach to specific proteins or nucleic acids.
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